We have developed novel wound-healing scaffolds that render the scaffolds themselves resistant to contamination with biofilms and enhances healing in difficult to treat patients.
Specifically, novel electrospinning methods have been developed to form protein scaffolds doped with variable amounts of our novel antimicrobial materials. The scaffolds have also been validated and verified to remain efficacious via in vitro antibacterial assays against two representative pathogens (Pseudomonas aeruginosa and Enterococcus sp.). Human dermal fibroblast cells are observed to nucleate and proliferate on scaffolds containing effective concentrations of the antimicrobials. These scaffolds alone support rapid wound closure and healing; however, they can also be used as a platform for the delivery of therapeutic agents to further promote wound healing (e.g., stem cell delivery, growth factor delivery, platelet-rich plasma activation/delivery). These scaffolds are fabricated using native skin proteins with integrated antimicrobials and, as a result, they more closely match the composition and architecture of skin, thereby enhancing wound healing while also inhibiting bacterial infection.